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Heart 109 , 686694 (2023)

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Ras is a family of related proteins called GTPases that function as molecular switches regulating pathways responsible for cell growth, proliferation, differentiation, and survival

Subsequently, the cells were fixed with 4% paraformaldehyde at 16 C for 15 min, permeabilized in phosphate buffered saline (PBS) with 0.1% Triton X-100 for 5 min at 16 C, and incubated with first antibodies at 4 C overnight, followed by incubation with FITC-conjugated secondary antibodies at 16 C for 1 h

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